Education and Experience

After completing my undergraduate degree in biochemistry, I began my Master's Degree at Queen's University in the Department of Pathology and Molecular Medicine, where I pursued my interests to combine computing and cell biology, and to study their applications in clinical science. I have since upgraded to the Doctoral Program, where I study under the supervision of Dr. Neil Renwick.

During my post-secondary education, I have had the opportunity to gain experience in a variety of clinical and laboratory settings, including SickKids hospital, the University of Toronto, the Banting and Best Diabetes Centre and the Rockefeller University, in addition to my primary research projects at Queen's.

Justin Wong CV

Research

My PhD project investigates the genetic nature of neuroendocrine tumors (NETs). Specifically, can microRNA (miRNA) profiling be used to classify and diagnose NETs? Furthermore, can these miRNA profiles explain the unique behaviour and morphology of NETs? I use next-generation (next-gen) sequencing, machine learning, and gene editing to discover novel biomarkers and oncogenes.

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Next-gen sequencing

miRNA are extracted from blood, FFPE tissue blocks, and cell lines, and sequenced with an Illumina Hi-Seq next-gen sequencer. The miRNA are then annotated and compiled into profiles for each individual patient.

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Machine learning

The miRNA profiles are analyzed with data mining and pattern recognition algorithms. These algorithms show the genetic relationships between subgroups of NETs. They also identify important features / genetic biomarkers to classify the NET subgroups and differentiate between cancerous and non-cancerous cells.

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Gene editing

Gene editing techniques such as CRISPR-Cas9 and lentiviral transfection are used to overexpress and knock down important miRNA biomarkers in NETs to determine their biological role.

Publications

Xiaojing Yang, Jina Nanayakkara, Duncan Claypool, Sadegh Saghafinia, Justin JM Wong, Minqi Xu, Xiantao Wang, Christopher JB Nichol, Iacovos P Michael, Markus Hafner, Xiaolong Yang, Neil Renwick
Scientific Reports, 11, 10455(2021), May 2021, doi: 10.1038/s41598-021-89855-4

Justin JM Wong, Paula S. Ginter, Kathrin Tyryshkin, Xiaojing Yang, Jina Nanayakkara, Zier Zhou, Thomas Tuschl, Yao-Tseng Chen, Neil Renwick
Cancers, 12(9), 2653, September 2020, doi: 10.3390/cancers12092653

Jina Nanayakkara, Kathrin Tyryshkin, Xiaojing Yang, Justin JM Wong, Kaitlin Vanderbeck, Paula S Ginter, Theresa Scognamiglio, Yao-Tseng Chen, Nicole Panarelli, Nai-Kong Cheung, Frederike Dijk, Iddo Z Ben-Dov, Michelle Kang Kim, Simron Singh, Pavel Morozov, Klaas E A Max, Thomas Tuschl, Neil Renwick
NAR Cancer, 2(3):1-12, September 2020, doi: 10.1093/narcan/zcaa009

Nicole Panarelli, Kathrin Tyryshkin, Justin JM Wong, Adrianna Majewski, Xiaojing Yang, Theresa Scognamiglio, Michelle Kang Kim, Kimberley Bogardus, Thomas Tuschl, Yao-Tseng Chen, Neil Renwick
Endocrine-Related Cancer, 29(1):47-57, January 2019, doi: 10.1530/ERC-18-0244

Other Interests

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Cooking
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Fencing
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Gymnastics
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Guitar
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Swing dancing